Journal: PLoS ONE

Article Title: The Interaction between Circulating Complement Proteins and Cutaneous Microvascular Endothelial Cells in the Development of Childhood Henoch-Schönlein Purpura

doi: 10.1371/journal.pone.0120411

Figure Lengend Snippet: The expression of chemokines and adhesion molecules by C3a/C5a treated-HMVEC-d.

Article Snippet: These cells were then stained by adding APC-conjugated mouse anti-human E-selectin monoclonal antibody (BD Biosciences) or PE-conjugated mouse anti-human ICAM-1 (CD54) monoclonal antibody (Serotec, USA) at 4°C for one hr.

Techniques: Expressing

Journal: PLoS ONE

Article Title: The Interaction between Circulating Complement Proteins and Cutaneous Microvascular Endothelial Cells in the Development of Childhood Henoch-Schönlein Purpura

doi: 10.1371/journal.pone.0120411

Figure Lengend Snippet: HMVEC-d were stimulated by C3a, C5a of different concentrations (0, 50, 100, 200 ng/ml), or TNF-α (10 ng/ml). Six hr and 18 hr later, the cells were harvested and analyzed for the expression of E-selectin (A) and ICAM-1 (B) by flow cytometry. The expression levels were presented as mean fluorescence intensity (MFI). * denotes p < 0.05, compared with the value of nontreated.

Article Snippet: These cells were then stained by adding APC-conjugated mouse anti-human E-selectin monoclonal antibody (BD Biosciences) or PE-conjugated mouse anti-human ICAM-1 (CD54) monoclonal antibody (Serotec, USA) at 4°C for one hr.

Techniques: Expressing, Flow Cytometry, Fluorescence

Journal: PLoS ONE

Article Title: The Interaction between Circulating Complement Proteins and Cutaneous Microvascular Endothelial Cells in the Development of Childhood Henoch-Schönlein Purpura

doi: 10.1371/journal.pone.0120411

Figure Lengend Snippet: * The expression of IL-8, MCP-1, E-selectin, and ICAM-1 by HMVEC-d was majorly enhanced by C5a.

Article Snippet: These cells were then stained by adding APC-conjugated mouse anti-human E-selectin monoclonal antibody (BD Biosciences) or PE-conjugated mouse anti-human ICAM-1 (CD54) monoclonal antibody (Serotec, USA) at 4°C for one hr.

Techniques: Expressing